说法网
CD4+T淋巴细胞穿孔素基因启动子区低甲基化状态在COPD中的作用摇滚狂潮
目的 探讨慢性阻塞性肺疾病（COPD）患者CD4+T 淋巴细胞穿孔素基因启动子区甲基化状态在该病中的作用。方法 分别分离8例COPD组和8例正常对照组外周静脉血CD4+T淋巴细胞，提取RNA和DNA，检测CD4+T 细胞穿孔素基因启动子区域 DNA 平均甲基化水平以及穿孔素基因mRNA的表达。结果 COPD患者CD4+T淋巴细胞穿孔素基因启动子区域DNA平均甲基化水平显著低于正常对照组（P﹤0.05），但穿孔素基因mRNA的表达较正常对照组增强（P﹤0.05）。结论 COPD患者CD4+T淋巴细胞穿孔素基因启动子区域DNA低甲基化状态可引起穿孔素基因mRNA的过度表达，在COPD发病中具有重要的作用。borland c 3.1
中国证监会微博Abstract：Objective This study aimed at investigating the methylation status of perforin regulatory elements in CD4+ T cells from peripheral blood of COPD patients and its possible role in the pathogenesis of this disease. Methods The peripheral blood CD4+ T lymphocytes of eight patients with COPD and eight healthy controls were separated， and genomic DNA and RNA was extracted from the isolated cells. The methylation levels of perforin regulatory elements in peripheral blood CD4+ T lymphocytes were determined， e
十字军东征的影响
可再生能源定额站xpression of perforin investigated.Results The methylation levels in CD4+T lymphocyte perforin regulatory elements of patients with COPD were significantly lower than those in the control group， while the expression levels of perforin gene were relatively higher and the difference was statistically significant （P <0.05）.Conclusion Perforin regulatory elements of CD4+ T lymphocyte are hypomethylated in COPD patients， which activates perforin gene involoved in the development of this disease.
