线路转换器
电厂脱硫
滤布废橡胶炼油Profiling Human Serum Antibodies with a Carbohydrate Antigen Microarray 期刊名称： Journal of Proteome Research电源外壳
作者： Oyindasola,Oyelaran,Lisa,M.,McShane,Lori,Dodd,Jeffrey,C.,Gildersleeve 作者机构： Laboratory
年份： 2009年
期号： 第9期
关键词： Glycan array; carbohydrate antigens; serum antibodies; microarray;
间硝基苯甲酸variability; normalization
摘要：Carbohydrate antigen arrays (glycan arrays) have been recently developed for the high-throughput analysis of carbohydrate macromolecule interactions. When profiling serum, information about experimental variability, interindividual biological variability, and intraindividual temporal variability is critical. In this report, we describe the characterization of a carbohydrate antigen array and assay for
profiling human serum. Through optimization of assay conditions and development of a normalization strategy, we obtain highly reproducible results with a within-experiment coefficient of variation (CV) of 10.8% and an overall CV (across multiple batches of slides and days) of 28.5%. We also report antibody profiles for 48 human subjects and evaluate for the first time the effects of age, race, sex, geographic location, and blood 防漏杯盖
