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14 ·2022.08遵义市委书记廖少华Experimental research | 试验研究2.2 回归检测试验步骤(1)使用微量移液器在96孔V 型微量反应板的1~4中加入0.025 mL PBS ,形成4个平行样本并更换滴头。(2)吸取经过均匀混合的4HAU 抗原0.025 mL 加入第1孔,再次摇匀。从第1孔中吸取0.025 mL 滴入第2孔中,再次摇匀,从第2孔中同样吸取0.025mL